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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
09/01/1997 |
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Data da última atualização: |
03/04/2017 |
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Autoria: |
DIRIE, M. E.; MURPHY, N. B.; GARDINER, P. R. |
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Título: |
DNA fingerprinting of Trypanosoma vivax isolates rapidly identifies intraspecific relationships. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Journal of Eukaryotic Microbiologie, v.40, n.2, p.132-134, 1993. |
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Idioma: |
Inglês |
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Conteúdo: |
Using the polymerase chain reaction and arbitrarily selected oligonucleotide primers of 10 or 11 bases, we have amplified DNA sequences from Trypanosoma vivax parasites isolated from South America and Africa. On the basis of polymorphisms in the DNA fingerprints generated by three of the primers, the parasite could be separated into two major groups, one comprising T. vivax isolates from Kenya and the second including all the other T. vivax parasites (from Colombia, The Gambia, Nigeria and Uganda). One of these three primers (ILo 525) also gave isolate-specific DNA fingerprints for the parasites tested, which will allow the use of this technique both in the species identification and discrimination of T. vivax parasites. |
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Palavras-Chave: |
Arbitrary primers; East Africa; Parasita; Parasite; West Afric. |
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Thesagro: |
Trypanosoma Vivax. |
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Thesaurus Nal: |
Colombia; polymerase chain reaction. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01415naa a2200241 a 4500
001 1790061
005 2017-04-03
008 1993 bl --- 0-- u #d
100 1 $aDIRIE, M. E.
245 $aDNA fingerprinting of Trypanosoma vivax isolates rapidly identifies intraspecific relationships.
260 $c1993
520 $aUsing the polymerase chain reaction and arbitrarily selected oligonucleotide primers of 10 or 11 bases, we have amplified DNA sequences from Trypanosoma vivax parasites isolated from South America and Africa. On the basis of polymorphisms in the DNA fingerprints generated by three of the primers, the parasite could be separated into two major groups, one comprising T. vivax isolates from Kenya and the second including all the other T. vivax parasites (from Colombia, The Gambia, Nigeria and Uganda). One of these three primers (ILo 525) also gave isolate-specific DNA fingerprints for the parasites tested, which will allow the use of this technique both in the species identification and discrimination of T. vivax parasites.
650 $aColombia
650 $apolymerase chain reaction
650 $aTrypanosoma Vivax
653 $aArbitrary primers
653 $aEast Africa
653 $aParasita
653 $aParasite
653 $aWest Afric
700 1 $aMURPHY, N. B.
700 1 $aGARDINER, P. R.
773 $tJournal of Eukaryotic Microbiologie$gv.40, n.2, p.132-134, 1993.
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Embrapa Pantanal (CPAP) |
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